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A consensus document for the clinical management of invasive fungal diseases in pediatric patients with hematologic cancer and/or undergoing hematopoietic stem cell transplantation in Brazilian medical centers BJID
Carlesse,Fabianne; Daudt,Liane Esteves; Seber,Adriana; Dutra,Álvaro Pimenta; Melo,Analy Salles de Azevedo; Simões,Belinda; Macedo,Carla Renata Donato; Bonfim,Carmem; Benites,Eliana; Gregianin,Lauro; Batista,Marjorie Vieira; Abramczyk,Marcelo; Tostes,Vivian; Lederman,Henrique Manoel; Lee,Maria Lúcia de Martino; Loggetto,Sandra; Castro Junior,Cláudio Galvão de; Colombo,Arnaldo Lopes.
ABSTRACT In the present paper we summarize the suggestions of a multidisciplinary group including experts in pediatric oncology and infectious diseases who reviewed the medical literature to elaborate a consensus document (CD) for the diagnosis and clinical management of invasive fungal diseases (IFDs) in children with hematologic cancer and those who underwent hematopoietic stem-cell transplantation. All major multicenter studies designed to characterize the epidemiology of IFDs in children with cancer, as well as all randomized clinical trials addressing empirical and targeted antifungal therapy were reviewed. In the absence of randomized clinical trials, the best evidence available to support the recommendations were selected. Algorithms for early...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungal infections; Children; Cancer.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000600395
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Identification of genes differentially expressed in hyphae of Candida albicans BJM
Melo,Analy Salles de Azevedo; Serafim,Rui Cosme; Briones,Marcelo Ribeiro da Silva.
The ability to switch from yeast to hyphal forms is essential for Candida albicans virulence. This morphological switch involves the expression of hyphal-specific genes under the control of transcriptional factors. To contribute to the discovery of hyphal-specific genes, we used a differential screening method where clones of a genomic DNA library were hybridized with yeast and hyphal cDNA probes. Two clones with increased expression in hyphae were selected for study. Sequencing these clones, we found that they encoded two important metabolic genes, CaHXT7 (high-affinity hexose transporter) and CaYLL34 (member of the AAA ATPase family). CaHXT7 and CaYLL34 ORFs were completely determined. Analyses of the putative proteins show that: (1) CaHxt7p has one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential screening; Candida albicans; HXT7; YLL34.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500046
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